Method for obtaining a culture medium from plant sap

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12P 7/40 (2006.01) C12P 13/04 (2006.01)

Patent

CA 2102528

2102528 9219716 PCTABS00017 The procedure involves heat treatment of the plant juice, first in a temperature x time interval from 55 ·C x 24 hours to 120 ·C x 10 min., whereafter the plant juice is cooled to 50 ·C - 60 ·C and the pH value is set at 7.5 - 8.5 by addition of a base. Hereafter the plant juice is converted by enzymic hydrolysis by means of proteolytic enzymes such as proteases and peptidases, while keeping the pH value constant at 7.5 - 8.5 by continuous addition of base until the hydrolysis is over. By the procedure a medium is formed which is well suited as a nutritive substrate for vitamin and amino acid demanding micro-organisms, which form organic acids or amino acids. By the procedure it is possible in a cheap and simple manner to convert plant juice to organic acids or amino acids.

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