Method for obtaining a purified viral preparation

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 7/02 (2006.01) B01D 15/08 (2006.01) B01J 8/18 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2364934

The invention concerns a method for purifying a crude viral preparation containing viral, in particular adenoviral, particles of interest. The invention is characterised in that it comprises a step of adsorption on a fluidised bed. The invention also concerns a protocol for producing viral particles for use in gene therapy comprising such a purifying process.

La présente invention concerne un procédé de purification d'une préparation virale brute renfermant des particules virales d'intérêt, notamment adénovirales, caractérisé en ce qu'il comporte au moins une étape d'adsorption en lit fluidisé. Elle a également pour objet un protocole pour la production de particules virales utilisables pour la thérapie génique comprenant un tel procédé de purification.

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