Method for obtaining a titrated preparation of algae and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) G01N 33/50 (2006.01) A61K 35/80 (2000.01)

Patent

CA 2453534

The invention concerns the field of chemistry and more particularly that of plant chemistry. The invention relates to a method for obtaining powders or extracts of the Padina pavonica alga, titrated in active principles by an equivalence ratio with estradiol, which consists in proportioning the Padina pavonica extract or powder using a method which comprises culturing osteoblasts in a culture medium rich in calcium ions, analysing the calcium fixed by the cells of the extracellular matrix in the presence or in the absence of agents antagonistic to the fixation of calcium and expressing the results either in arbitrary or international units relative to a reference standard, either with reference to the known activity of specific doses of estradiol or any other agent promoting the fixation of calcium. The invention is for use in biology.

La présente invention se rapporte à un procédé d'obtention de poudres ou d'extraits de l'algue Padina pavonica, titrés en principes actifs par l'intermédiaire d'un coefficient d'équivalence avec l'estradiol, qui consiste à doser l'extrait ou la poudre de Padina pavonica à l'aide d'une méthode dans laquelle on cultive des ostéoblastes dans un milieu de culture riche en ions calcium, on analyse le calcium fixé par les cellules de la matrice extracellulaire en présence ou en l'absence d'agents antagonistes de la fixation du calcium et on exprime les résultats soit en unités arbitraires ou intemationales par rapport à un étalon de référence, soit par référence avec l'activité connue de doses déterminées d'estradiol ou de tout autre agent favorisant la fixation du calcium.

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