A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
167/135
A61K 39/095 (2006.01) A61K 39/00 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07K 14/22 (2006.01) C07K 14/52 (2006.01) C07K 16/12 (2006.01) C12N 9/00 (2006.01)
Patent
CA 1341199
A method is provided for obtaining a vaccine against the different pathogenic serotypes of group B Neisseria meningitidis characterized by starting from live microorganisms of any one of the known pathogenic serotypes of the B group without inactivation, from which the extractation of the vesicles of the outer membrane and the protein antigenic complex of 65 - 95 KD molecular weight is carried out using detergent, enzyme and ultrasound combined in the treatment. The resulting product, after treatment to eliminate the nucleic acids, is purified by a dissociating treatment with detergent, ultrasonic bath and column chromatography. The multi-antigenic material so obtained is purified to obtain the protein antigenic complex of 65-95 KD molecular weight for further purification by HPLC chromatography (TSK 3000 SWG column) or affinity chromatography with monoclonal antibodies, or hydrophobicity chromatography, or ionic exchange chromatography or a combination of any two or more of them. The protein antigenic complex is then added to a solution that contains vesicles in a proportion of 15 percent ~ 3. The protein antigenic complex is then anchored to the vesicles by ultrasound treatment. The capsular polysaccharide is also added, in a proportion of 1.1 - 1.4 with respect to the protein and the adjuvant in a relation ranging from 20 - 100 mcg/protein mcg. The different components of the mixture may be sterilized by cobalt 60 ionizing radiations with doses from 5 - 25 Kgy and a temperature between 1 - 4°C before preparing the mixture or the resulting mixture may be sterilized by this procedure.
573538
Campa Huergo Concepcion
Dominguez Manuel Alfredo Galguera
Gonzalez Victoriano Gustavo Sierra
Herrera Maria Del Carmen Sampedro
Imia Luis Guillermo Garcia
Centro Nacional de Biopreparados
Instituto Finlay
Macrae & Co.
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