C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/12 (2006.01) C07K 14/48 (2006.01)
Patent
CA 2346257
The invention relates to a method for producing biologically active .beta.-NGF from the proform proNGF. After expressing the proform of the .beta.-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural .beta.-NGF, biologically active .beta.-NGF is obtained by subsequently splitting-off the prosequence.
L'invention concerne un procédé de préparation de NGF- beta biologiquement actif, à partir de la proforme proNGF. Après expression de la proforme du NGF- beta dans une cellule hôte procaryote, la protéine de recombinée est isolée sous forme d'ensembles inactifs insolubles (corps d'inclusion). Après leur solubilisation dans un agent de dénaturation puissant puis leur conversion à la conformation naturelle qui est déterminée par les ponts disulfure présents dans le NGF- beta naturel, le NGF- beta biologiquement actif est obtenu par séparation de la proséquence.
Grossmann Adelbert
Rattenholl Anke
Rudolph Rainer
Schwarz Elisabeth
Oyen Wiggs Green & Mutala Llp
Scil Proteins Gmbh
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