C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C07K 14/47 (2006.01) C12N 9/16 (2006.01) C12N 15/62 (2006.01) C12Q 1/42 (2006.01)
Patent
CA 2394152
The invention concerns a method for obtaining human cdc 25B1, cdc25B2, cdcB3 and cdc25C phosphatases. More particularly, the invention concerns a protein for fusion between human cdc25B1, cdc25B2, cdcB3 or cdc25C phosphatase and the maltose binding protein (MBP) of Escherichia coli, the DNA sequence coding for said fusion protein, a method for preparing said fusion protein and a method for identifying human cdc25B1, cdc25B2, cdcB3 or cdc25C protein modulators.
La présente invention concerne une méthode d'obtention de phosphatases humaines cdc25B1, cdc25B2, cdcB3 et cdc25C. L'invention a en particulier pour objet une protéine de fusion entre la phosphatase humaine cdc25B1, cdc25B2, cdcB3 ou cdc25C et la protéine liant le maltose (Maltose Binding Protein ou MBP) d'Escherichia coli, la séquence d'ADN codant pour ladite protéine de fusion, un procédé de préparation de ladite protéine de fusion ainsi qu'une méthode d'identification de modulateurs de la protéine cdc25B1, cdc25B2, cdcB3 ou cdc25C humaine.
Ducommun Bernard
Goubin-Gramatica Francoise
Prevost Gregoire
Goudreau Gage Dubuc
Societe de Conseils de Recherches Et D'applications Scientifique
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