Method for obtaining human smooth muscular cells and uses...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/077 (2010.01) A61K 35/12 (2006.01) A61K 35/34 (2006.01)

Patent

CA 2622916

The invention concerns a method for obtaining in vitro a population of cells comprising essentially human smooth muscular cells expressing calponin and SM-MHC from a sample of human muscular biopsy or from human muscular biopsies differentiated in vitro into skeletal muscle cells. The invention also concerns a composition comprising the isolated smooth muscular cells obtainable by said method as therapeutic principle designed for humans. The invention further concerns the use of the isolated smooth muscular cells for preparing a therapeutic composition designed to replace smooth muscular cells. In particular, the invention concerns the use of said isolated smooth muscular cells for treating ischemia, cancer or any disease requiring revascularization of damaged tissues. Finally, the invention concerns the use of said smooth muscular cells as vector for an active principle for preparing a therapeutic composition designed for humans requiring treatment with said active principle.

La présente invention est relative à un procédé d'obtention in vitro d'une population de cellules comprenant essentiellement des cellules musculaires lisses humaines (hCML) exprimant la calponine et la SM-MHC à partir d'un échantillon de cellules de biopsie musculaire humaine ou à partir de cellules de biopsies musculaires humaines différenciées in vitro en cellules musculaires squelettiques (hCMS). L'invention comprend également une composition comprenant les cellules musculaires lisses isolées susceptibles d'être obtenues par un tel procédé à titre de composition thérapeutique destinée à l'Homme. L'invention a également pour objet l'utilisation de ces cellules musculaires lisses isolées pour la préparation d'une composition thérapeutique destinée au remplacement de CMLs. En particulier, l'invention a pour objet l'utilisation de ces cellules musculaires lisses isolées pour le traitement de l'ischémie, du cancer ou de toute maladie nécessitant la revascularisation de tissus endommagés. Enfin, la présente invention a pour objet l'utilisation de ces cellules musculaires lisses isolées comme vecteur de principe actif pour la préparation d'une composition thérapeutique destinée à l'homme nécessitant un traitement par ce principe actif.

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