Method for obtaining in vitro recombined polynucleotide...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C07K 14/00 (2006.01) C07K 14/245 (2006.01) C12N 1/21 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2339901

The invention concerns a method for producing in vitro recombined polynucleotide sequences from a bank of polynucleotide sequences, characterised in that it comprises the following steps: fragmenting an initial bank of double-strand polynucleotide sequences; (b) denaturation of the fragments resulting from step (a) optionally in the presence of one or several assembling matrix or matrices; (c) hybridising said fragments with one or several of the assembling matrix or matrices if the latter is/are not present in step (b); (d) ligation of said fragments; (e) optionally cloning said recombined polynucleotide sequences having advantageous properties with respect to one or several reference sequences.

La présente invention a pour objet un procédé de production in vitro de séquences polynucléotidiques recombinées à partir d'une banque de séquences polynucléotidiques, caractérisé en ce qu'il comprend les étapes suivantes: (a) la fragmentation d'une banque initiale de séquences polynucléotidiques double- brins; (b) la dénaturation des fragments issus de l'étape (a) éventuellement en présence d'une ou plusieurs matrice(s) d'assemblage; (c) l'hybridation desdits fragments avec une ou plusieurs matrice(s) d'assemblage si celle(s)-ci n'est (ne sont) pas présente(s) à l'étape (b); (d) la ligation desdits fragments; (e) éventuellement le clonage des séquences polynucléotidiques recombinées, et la sélection des séquences polynucléotidiques recombinées, et la sélection des séquences polynucléotidiques présentant des propriétés avantageuses par rapport à une ou plusieurs séquences de référence.

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