Method for obtaining nucleic acids from an environment...

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 19/34 (2006.01) C07K 16/40 (2006.01) C12N 9/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2393041

The invention concerns a method for preparing nucleic acids from an environment sample, more particularly a method for obtaining a library of nucleic acids from a sample. The invention also concerns nucleic acids of nucleic acid libraries obtained by said method their use in the synthesis of novel compounds, in particular novel compounds of therapeutic interest. The invent further concerns novel means used in the method for obtaining said nucleic acids, such as novel vectors and novel processes for preparing such vectors or recombinant host cells containing said nucleic acid. Finally, the invention concerns methods for detecting a nucleic acid of interest within a library of nucleic acids resulting from said method, and nucleic acids detected by said method and polypeptides encoded by said nucleic acids.

La présente invention concerne un procédé de préparation d'acides nucléiques à partir d'un échantillon de l'environnement, plus particulièrement un procédé d'obtention d'une collection d'acides nucléiques à partir d'un échantillon. L'invention est également relative aux acides nucléiques ou aux collections d'acides nucléiques obtenus selon le procédé et leur application à la synthèse de nouveaux composés, notamment de nouveaux composés d'intérêt thérapeutique. L'invention a également pour objet les moyens nouveaux mis en oeuvre dans le procédé d'obtention d'acides nucléiques ci-dessus, tels que de nouveaux vecteurs et des nouveaux procédés de préparation de tels vecteurs ou encore des cellules hôtes recombinantes comprenant un acide nucléique de l'invention. L'invention concerne encore des procédés pour détecter un acide nucléique d'intérêt au sein d'une collection d'acides nucléiques obtenus selon le procédé ci-dessus, ainsi que les acides nucléiques détectés par un tel procédé et les polypeptides codés par de tels acides nucléiques.

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