C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/09 (2010.01) C12Q 1/02 (2006.01)
Patent
CA 2603133
The present invention relates to methods for obtaining primary-culture tumour cells from tumour tissue. More precisely, the present invention relates to a method for obtaining primary-culture tumour cells from tumour tissue, in particular breast carcinoma, where, in one method step, the tumour tissue is divided into tumour-tissue sections of a certain size, and these tissue sections are then cultured under defined conditions. The invention also concerns the primary-culture tumour cells that can be obtained by this method from the tumour tissue, in particular primary-culture tumour cells from breast carcinoma. Finally, the invention relates to the use of these primary-culture tumour cells for, among other things, determining an individual course of tumour treatment, or for testing and screening of new therapeutic agents against tumours.
L'invention concerne des procédés permettant de produire des cellules tumorales de culture primaire à partir de tissus tumoraux. L'invention concerne plus précisément un procédé permettant de produire des cellules tumorales de culture primaire à partir de tissus tumoraux, notamment de carcinome mammaire. Dans une première étape du procédé, le tissu tumoral est fractionné en fragments de tissu tumoral de dimension déterminée et ces fragments de tissu sont ensuite mis en culture, dans des conditions déterminées. L'invention concerne en outre les cellules tumorales de culture primaire pouvant être obtenues à l'aide dudit procédé, à partir de tissus tumoraux, notamment des cellules tumorales de culture primaire issues du carcinome mammaire. L'invention concerne pour finir l'utilisation de ces cellules tumorales de culture primaire, entre autres pour déterminer une thérapie tumorale individuelle ou pour tester et sélectionner de nouveaux agents thérapeutiques tumoraux.
Dehn Axel
Hass Ralf
Kueppers Hartmut
Rogoll Jutta
Kirby Eades Gale Baker
Medizinische Hochschule Hannover
Solvay Arzneimittel Gmbh
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