Method for opening tight junctions

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01)

Patent

CA 2702180

The present invention is directed to a method and use of RNA interference (RNA1) for the transient, reversible and controlled opening of the tight junctions of the blood brain barrier and/or the blood retinal barrier. This method may be used in the treatment of many diseases and disorders which require the opening of the blood brain barrier and/or blood retinal barrier. Such methods generally involve the use of an RNA1-inducing agent, such as s1RNA, m1RNA, shRNA or an RNA1-inducing vector whose presence within a cell results in production of an s1RNA or shRNA, targeting tight junction proteins to open the blood brain barrier and/or blood retinal barrier.

La présente invention porte sur un procédé et sur l'utilisation d'une interférence d'ARN (ARNi) pour l'ouverture transitoire, réversible et contrôlée des jonctions serrées de la barrière hémato-encéphalique et/ou de la barrière hémato-rétinienne. Ce procédé peut être utilisé dans le traitement de nombreuses maladies et de nombreux troubles qui nécessitent l'ouverture de la barrière hémato-encéphalique et/ou de la barrière hémato-rétinienne. De tels procédés mettent en jeu, d'une manière générale, l'utilisation d'un agent induisant l'ARNi, tel que l'ARNsi, l'ARNmi, l'ARNsh, ou un vecteur induisant l'ARNi dont la présence à l'intérieur d'une cellule conduit à la production d'un ARNsi ou d'un ARNsh, ciblant des protéines de jonctions serrées pour ouvrir la barrière hémato-encéphalique et/ou la barrière hémato-rétinienne.

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