Method for ordering macromolecules by means of a moving...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07K 1/04 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01) G01N 33/544 (2006.01)

Patent

CA 2182905

A method for ordering one or more macromolecules on the surface (S) of a support, wherein a triple line S/A/B (meniscus) resulting from a solvent (A) contacting the surface (S) and a medium (B) is moved over said surface, said macromolecules having a portion, particularly one end, anchored to the surface (S), and another portion, or the other end, dissolved in the solvent (A). A method for revealing, measuring the intramolecular distance of, separating and/or assaying a macromolecule in a sample in which said ordering method is carried out, is also disclosed.

La présente invention a pour objet un procédé d'alignement de macromolécule(s) sur la surface (S) d'un support, caractérisé en ce que l'on fait se déplacer sur ladite surface (S) la ligne triple S/A/B (ménisque) résultant du contact d'un solvant (A) avec la surface (S) et un milieu (B), lesdites macromolécules ayant une partie, notamment une extrémité, ancrée sur la surface (S), l'autre partie, notamment l'autre extrémité, étant en solution dansle solvant (A). La présente invention a également pour objet un procédé de mise en évidence, de mesure de distance intra-moléculaire, de séparation et/ou dosage d'une macromolécule dans un échantillon dans lequel on met en oeuvre un procédé d'alignement selon l'invention.

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