Method for performing and tracing enzymatic reactions and a...

C - Chemistry – Metallurgy – 12 – Q

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150/15

C12Q 1/00 (2006.01) C12M 1/40 (2006.01)

Patent

CA 1218289

ABSTRACT OF THE DISCLOSURE: An enzymatic material is immobilized onto the surface of a removable stirrer, e.g. magnetic or mechanical, or alternatively on a surface of another member which is removably fixed in a reactor. The enzymatic material may either be immobilized directly or indirectly onto the strir- rer or the other removable member. The enzymatic material is used in a quantity sufficient such that the concentration of the determined substance on the surface of the enzymatic layer may be maintained at a value of 0 to 3% during the course of a plurality of measurements. The enzymatic mate- rial may be fixed onto the surface of the stirrer of other removable member either directly or by means of a suitable layer, foil or cloth. The fixing of enzymatic material onto the stirrer or other removable member obviates all drawbacks of enzymatic electrodes, while providing the advantage of numerous applications of the immobilized enzyme, as well as specific determinations.

459834

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