Method for polymerising glycolic acid with microorganisms

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 7/62 (2006.01)

Patent

CA 2730220

The present invention relates to a method for producing and preparing polyglycolate (PGA) from genetically engi-neered organisms. More specifically, the invention relates to a method comprising two steps; 1) culturing, in a medium containing glycolic acid or not, the microorganism expressing at least one gene encoding an enzyme(s) that converts glycolate into glycolyl--CoA, and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses glycolyl-CoA as a substrate, 2) recovering the polyglycolate polymer.

La présente invention porte sur un procédé pour la fabrication et la préparation de polyglycolate (PGA) à partir d'organismes modifiés par génie génétique. De façon plus spécifique, l'invention porte sur un procédé comprenant deux étapes : 1) la culture, dans un milieu contenant ou non de l'acide glycolique, du micro-organisme exprimant au moins un gène codant pour une ou plusieurs enzymes qui convertissent le glycolate en glycolyl-CoA, et un gène codant pour la polyhydroxyalcanoate (PHA) synthase qui utilise la glycolyl-CoA en tant que substrat, 2) la récupération du polymère polyglycolate.

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