C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/04 (2006.01) C12N 1/14 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2575671
A method for preservation of a microorganism capable of microbial production of a polyunsaturated fatty acid or a compound comprising a polyunsaturated fatty acid as a constituent fatty acid, which method comprises: (a) forming spores in a spore-forming medium at pH 4-7 containing a nutrient source comprising an inorganic salt and a saccharide; (b) suspending the spores obtained in (a) in sterilized water, or sterilized water containing a surfactant and/or an inorganic salt to prepare a spore suspension, and adding a cryoprotectant at 5-15% to prepare a cryopreserving spore suspension; and (c) preserving the cryopreserving spore suspension obtained in (b) at between - 100~C and -20~C.
L'invention concerne un procédé de conservation d'un microorganisme capable d'effectuer une production microbienne d'un acide gras polyinsaturé ou d'un composé comprenant un acide gras polyinsaturé comme acide gras constituant, lequel procédé comprend: (a) la formation de spores dans un milieu de formation de spores à un pH de 4 à 7 contenant une source nutritive comprenant un sel inorganique et un saccharide; (b) la mise en suspension des spores obtenues dans (a) de l'eau stérilisée, ou de l'eau stérilisée contenant un agent tensioactif et/ou un sel inorganique pour préparer une suspension de spores, et l~ajout de 5 à 15 % de cryoprotecteur pour préparer une suspension de spores de cryoconservation; et (c) la conservation de la suspension de spores de cryoconservation obtenue en (b) à une température comprise entre -100°C et -20°C.
Nippon Suisan Kaisha Ltd.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Suntory Limited
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