Method for preparation and transplantation of volute grafts...

A - Human Necessities – 61 – F

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A61F 9/007 (2006.01) A61F 2/14 (2006.01) A61B 17/00 (2006.01)

Patent

CA 2158443

A method of transplant- ing a graft in the subretinal area of a host eye comprises prepar- ing the graft by harvesting from the donor tissue a population of cells in a manner that main- tains the population of cells in the same organization and cel- lular polarity as is present in normal tissue of that type. The population of cells are of a sheet-like from and are assem- bled with a relatively thin flex- ible pliable carrier composed of a non-toxic flexible compo- sition which substantially dis- solves at body temperature to form a graft. The graft is suf- ficiently flexible and pliable to be coiled to form a volute (76) without disturbing the organi- zation and polarity of the cells. The method further comprises coiling the graft to form a vo- lute (76) with the convolutions of the volute (76) free of one another for subsequent uncoiling of the graft substantially to its original sheet-like form. An incision is made in the host eye for insertion of the volute (76). The incision in the eye is smaller than the incision that would be required for insertion of the graft in its uncoiled sheet-like form. The volute is inserted one end first into the host eye through the incision and transported to a position between the retina and the underlying tissue. The volute (76) uncoils after its insertion to lie in sheet-like form between the retina and underlying tissue of the host eye. The incision is then closed.

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