Method for preparation of herpes simplex virus subunit vaccine

A - Human Necessities – 61 – K

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A61K 39/245 (2006.01) C07K 14/035 (2006.01) C12N 7/04 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1251401

Abstract of Disclosure A method for the preparation of an herpes simplex virus subunit vaccine, which comprises subjecting a solution containing glycoproteins gA and gB to column chromatography using, as a gel for chromatography, sulfuric acid ester of cellulose or a crosslinked polysaccharide to adsorb the glycoproteins gA and gB onto the gel in the presence of an anionic surfactant or a nonanionic surfactant, eluting the adsorbed glycoproteins to obtain an eluate containing the glycoproteins, and subjecting the eluate to gel filtration to obtain a filtrate containing the glycoproteins.

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