Method for preparation of single chain antibodies

Details Method for preparation of single chain antibodies Method for preparation of single chain antibodies

A - Human Necessities

61

K

A61K 39/395 (2006.01) A61K 47/48 (2006.01) A61K 49/00 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01) C07K 16/42 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2463017

This invention provides a method for identifying cells expressing a target single chain antibody (scFv) directed against a target antigen from a collection of cells that includes cells that do not express the target scFv, comprising the step of combining the collection of cells with an anti- idiotype directed to an antibody specific for the target antigen and detecting interaction, if any, of the anti-idiotype with the cells, wherein the Occurrence of an interaction identifies the cell as one which expresses the target scFv. This invention also provides a method for making a single chain antibody (scFv) directed against an antigen, wherein the 15 selection of clones is made based upon interaction of those clones with an appropriate anti- idiotype, and heretofore inaccessible scFv so made. This invention provides the above , methods or any combination thereof. Finally, this invention! provides various uses of these methods.

L'invention concerne un procédé permettant d'identifier des cellules exprimant un anticorps cible monocaténaire (scFv) dirigé contre un antigène cible, à partir d'une collection de cellules qui contient des cellules n'exprimant pas le scFv cible. Ce procédé comprend l'étape consistant à combiner la collection de cellules avec un anti-idiotype dirigé contre un anticorps spécifique de l'antigène cible et à détecter l'interaction, quand il y en a une, entre l'anti-idiotype et les cellules, l'apparition d'une interaction indiquant que la cellule est une cellule qui exprime le scFv cible. Cette invention concerne également un procédé de réalisation d'un anticorps monocaténaire (scFv) dirigé contre un antigène, la sélection de clones étant réalisée sur la base de l'interaction des clones présentant un idiotype approprié, ainsi que le scFv, qu'il était jusqu'ici impossible de réaliser, ainsi obtenu. Cette invention concerne les procédés susmentionnés ou n'importe quelle combinaison de ceux-ci. Enfin, cette invention concerne différentes utilisations de ces procédés.

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