Method for preparing a cartilaginous neo-tissue

Details Method for preparing a cartilaginous neo-tissue Method for preparing a cartilaginous neo-tissue

A - Human Necessities

61

L

A61L 27/38 (2006.01) A61L 27/20 (2006.01) A61L 27/52 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2442732

The invention concerns the preparation of a cartilaginous neo-tissue capable of being grafted. The method consists in: a) culturing chondrogenic cells, which are either autologous chondrocytes or cells precursors of chondrocytes prepared in vitro from pluripotent stem cells; b) contacting said chondrogenic cells with a chitosan hydrogel, having preferably a degree of acetylation ranging between 40 and 60 %, whereof the amphiphilic properties and the degree of acetylation is such that said cells adhere naturally to the outer surface of said hydrogel; c) covering the hydrogel/cell assembly thus obtained with a culture medium; and d) allowing a cartilaginous neo-tissue to develop in contact with the chitosan hydrogel during a minimum period of two weeks, frequently renewing the culture medium.

L'invention concerne la préparation d'un néo-tissu cartilagineux greffable. Le procédé consiste: a) à mettre en culture des cellules chondrogéniques, qui sont soit des chondrocytes autologues soit des cellules précurseurs de chondrocytes préparées in vitro à partir de cellules souches pluripotentes, b) à mettre lesdites cellules chondrogéniques en contact avec un hydrogel de chitosane-ayant de préférence un degré d'acétylation compris entre 40 et 60 % - dont les propriétés d'amphiphilie et le degré d'acétylation sont tels que lesdites cellules adhèrent naturellement à la surface extérieure dudit hydrogel, c) à recouvrir l'ensemble hydrogel/cellules ainsi obtenu par un milieu de culture et, d) à laisser se développer un néo-tissu cartilagineux au contact de l'hydrogel de chitosane pendant une durée minimale de deux semaines, en renouvelant fréquemment le milieu de culture.

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