Method for preparing a multicombinatorial library of...

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 15/62 (2006.01) C12N 15/10 (2006.01) C12N 15/13 (2006.01) C12P 21/02 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) C12N 7/01 (2006.01)

Patent

CA 2183079

On the basis of a first repertoire of genes coding for a population of one of two kinds of polypeptides capable of being combined, particularly an antibody light chain variable region, and at least one gene, and preferably a second repertoire coding for the other kind of polypeptide, particularly an antibody heavy chain variable region, the genes from the first repertoire are inserted into a first vector to form a vector population and the genes from the second repertoire are inserted into a second vector, at least one of said vectors being a recipient for the expression of both genes as polypeptides irreversibly combined with the outer surface of the product of said vector.

Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus. Partant d'un premier répertoire de gènes codant pour une population d'un de deux types de polypetides susceptibles de s'associer, notamment de région variable de chaîne légère d'anticorps, et d'au moins d'un gène et de préférence un second répertoire codant pour l'autre type de polypeptide, notamment une région variable de chaîne lourde d'anticorps, on introduit les gènes dudit premier répertoire dans un premier vecteur pour former une population de vecteurs et les gènes dudit second répertoire dans un second vecteur, l'un au moins desdits vecteurs, étant receveur pour exprimer les deux gènes sous forme de polypeptides associés à la surface extérieure du produit dudit vecteur de façon irréversible.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for preparing a multicombinatorial library of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for preparing a multicombinatorial library of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing a multicombinatorial library of... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1388793

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.