Method for preparing a protein comprising at least one...

Details Method for preparing a protein comprising at least one... Method for preparing a protein comprising at least one...

C - Chemistry, Metallurgy

12

N

C12N 15/26 (2006.01) C07K 1/107 (2006.01) C07K 1/113 (2006.01) C07K 14/55 (2006.01)

Patent

CA 2073564

Method for preparing a protein comprising at least one intramolecular disulphide bond, and possibly one or more additional cysteines, involving oxidation of the associated reduced recombinant protein by means of an oxidizing agent, and characterized in that an aqueous solution of the reduced protein is reacted at a pH of less than 5Ø

2073564 9209623 PCTABS00130 Procédé de préparation d'une protéine comportant au moins un pont disulfure intramoléculaire, et éventuellement une ou plusieurs cystéines supplémentaires, comprenant l'oxydation de la protéine recombinante réduite correspondante à l'aide d'un agent d'oxydation et caractérisé en ce que l'on fait réagir une solution aqueuse de la protéine réduite à un pH inférieur à 5,0.

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