Method for preparing an immobilized enzyme conjugate and...

Details Method for preparing an immobilized enzyme conjugate and... Method for preparing an immobilized enzyme conjugate and...

C - Chemistry, Metallurgy

12

N

C12N 11/00 (2006.01) C12N 11/14 (2006.01)

Patent

CA 2131225

Abstract of the Invention Method for Preparing An Immobilized Enzyme Conjugate And Immobilized Enzyme Conjugates Prepared Thereby A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.

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