Method for preparing an influenza virus, antigens obtained...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/06 (2006.01) A61K 39/145 (2006.01) C07K 14/11 (2006.01)

Patent

CA 2197683

Method for preparing purified influenza antigens from a fluid containing influenza virus, comprising the steps of concentration, purification, fragmentation and, where appropriate, inactivation, characterized in that: A. either the purification step entails several ultra-centrifugation steps separated by a filtration step, B. or the fragmentation step is performed on the live virus in the presence of an amphiphilic nonionic detergent, followed by a removal of undesirable substances by filtration, retaining all of the viral constituents, or these two steps are carried out in any order.

Procédé de préparation d'antigèns grippaux purifiés, à partir d'un liquide contenant le virus grippal, comprenant les étapes de concentration, de purification, de fragmentation et éventuellement d'inactivation caractérisé en ce que: A. <u>ou bien</u> l'étape de purification comporte plusieurs étapes d'ultracentrifugation séparées par une étape de filtration, B. <u>ou bien</u> l'étape de fragmentation est effectuée sur le virus vivant en présence d'un détergent non-ionique amphiphile suivie d'une élimination des substances indésirables par filtration retenant l'ensemble des constituants viraux, <u>ou bien</u> on opère selon ces deux étapes dans un ordre quelconque.

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