C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/074 (2010.01) C12N 5/071 (2010.01) A61K 35/12 (2006.01)
Patent
CA 2491043
The invention relates to tissue regeneration and repair by cell transplant. More particularly, the invention concerns a method for preparing adult stem cells which preserves the diversity and plasticity of said cells. The invention also concerns the use of said stem cells obtained by the inventive extraction method for therapeutic uses, including gene and cell therapy. The inventive preparation method essentially consists in: cell extraction, mechanical dissociation, enzymatic dissociation, maintaining the cells obtained in a specific culture medium enabling preservation of diversity and plasticity, said method excluding cell culture.
La présente invention se rapporte au domaine de la régénération et la réparation tissulaire par transplantation cellulaire. Plus particulièrement, la présente invention vise un procédé de préparation de cellules souches adultes qui préserve la diversité et la plasticité de ces cellules. La présente invention vise également l'usage de ces cellules souches obtenues par le procédé d'extraction proposé dans des applications thérapeutiques, dont la thérapie génique et cellulaire.Le procédé de préparation selon l'invention consiste essentiellement en : l'extraction cellulaire, la dissociation mécanique, la dissociation enzymatique, le maintien des cellules obtenues dans un milieu de culture spécifique permettant de préserver la diversité et la plasticité, ledit procédé excluant la mise en culture des cellules.
Borenstein Nicolas
Montarras Didier
Pinset Christian
Celogos
Centre National de La Recherche Scientifique
Fondation de L'avenir
Institut Pasteur
Robic
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