Method for preparing bioactive polymers

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 17/08 (2006.01) A61K 47/48 (2006.01) C07K 17/10 (2006.01) C12N 11/08 (2006.01) C12N 11/10 (2006.01)

Patent

CA 2260324

A method to prepare a bioactive polymer by covalently binding at least one amino group containing ligand to at least one polymer containing a plurality of free hydroxyl groups, said method comprising the following steps: (i) reacting the at least one polymer with an appropriate activating agent; (ii) reacting the resultant activated polymer in aqueous solution with desired amino group containing ligands; (iii) blocking by reaction or removing by hydrolysis residual polymer bound-ligand unreacted, activating groups; and wherein the said activating agent and/or leaving by-products formed by step (i) and/or by step (ii) and/or by step (iii), are swelling agents of the support polymer.

La présente invention concerne un procédé de préparation d'un polymère bioactif, en liant par covalence un groupe amine contenant un ligand, à un ou plusieurs polymères contenant un ensemble de groupes hydroxyles, ledit procédé comportant les étapes suivantes : (i) réaction du ou desdits polymères avec un agent activant approprié, (ii) réaction du polymère activé résultant dans une solution aqueuse avec le groupe amine voulu contenant des ligands, (iii) blocage des groupes d'activation par réaction ou retrait par hydrolyse du polymère résiduel non réagi par liaison du ligand. Selon le procédé, ledit agent activant et/ou les sous-produits résiduels de l'étape (i) et/ou de l'étape (ii) et/ou de l'étape (iii) sont des agents de gonflement du polymère support.

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