C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/139, 195/68
C12P 39/00 (2006.01) C12P 19/12 (2006.01) C12P 19/24 (2006.01)
Patent
CA 2027861
A method for preparing cellobiose from sucrose in a high yield at low cost without difficulty by using three enzymes of sucrose phosphorylase, glucose isomerase and cellobiose phosphorylase in the presence of orthophosphate. The additional method of the invention comprises 4 steps: to treat sucrose with sucrose phosphorylase in the presence of orthophosphate to produce fructose and glucose-1-phosphate; to treat the fructose obtained in the preceding step with glucose isomerase to produce glucose; to treat the glucose obtained in the preceding step and the glucose-1-phosphate obtained in the first step with cellobiose phosphorylase to produce cellobiose and orthophosphate; and to recover at least a part of cellobiose from the reaction mixture in the preceding step and to recycle at least a part of the remaining reaction mixture containing orthophosphate to the first step.
Kitaoka Motomitsu
Sasaki Takashi
Taniguchi Hajime
Forestry And Fisheries Director-General Of National Food Research Institute Ministry Of
Gowling Lafleur Henderson Llp
Nippon Petrochemicals Company Limited
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