Method for preparing conditioned medium of astrocyte-like cells

Details Method for preparing conditioned medium of astrocyte-like cells Method for preparing conditioned medium of astrocyte-like cells

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/079 (2010.01) C12N 5/0793 (2010.01)

Patent

CA 2578685

Disclosed are a method for preparing a conditioned medium of astrocyte-like cells derived from embryonic stem cells, characterized in that astrocyte-like cells, which are prepared by differentiation of embryonic stem cells, are cultured; the medium obtained by said method, the use of the conditioned medium for culturing neural cells as well as for inducing the differentiation of embryonic stem cells into neural cells.

La présente invention concerne un procédé de fabrication d'un milieu d'adaptation pour des cellules de type astrocyte provenant de cellules souches embryonnaires, caractérisé par la culture de cellules de type astrocyte obtenues par la différentiation de cellules souches embryonnaires ; un milieu obtenu par ce procédé ; et l'utilisation de ce milieu pour la culture de cellules nerveuses et l'induction de la différentiation de cellules souches embryonnaires dans des cellules neurales et ainsi de suite.

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