C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2344468
An RecA-like recombinase/single-stranded nucleic acid probe/double-stranded target nucleic acid complex can be very efficiently and specifically formed by a method comprising preparing an RecA-like recombinase/single-stranded nucleic acid probe complex in the presence of a hardly degradable nucleotide cofactor which has molecules in a number 1/4 times or more larger than the number of the molecules contained in the nucleotide residues constituting the single- stranded nucleic acid probe but 10 times or less larger than the number of the molecules of the RecA-like recombinase, and bringing the obtained complex with a specimen containing the double-stranded target nucleic acid.
On peut élaborer très efficacement et spécifiquement un complexe sonde à l'acide nucléique monocaténaire/recombinase de type RecA/acide nucléique cible bicaténaire en utilisant un procédé qui consiste à élaborer un complexe sonde à l'acide nucléique monocaténaire/recombinase de type RecA en présence d'un cofacteur nucléotidique difficilement dégradable ayant des molécules dont le nombre est supérieur d'un quart au nombre de molécules contenues dans les résidus nucléotidiques qui constituent la sonde à l'acide nucléique monocaténaire, mais supérieur de 10 fois ou moins au nombre de molécules de la recombinase de type RecA. Ensuite, on combine ce complexe avec un échantillon renfermant l'acide nucléique cible bicaténaire.
Kigawa Koji
Kusumi Kayo
Mukai Eli
Obata Kazuaki
Daikin Industries Ltd.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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