Method for preparing macrophages, and kits and compositions...

A - Human Necessities – 61 – K

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Details

A61K 35/14 (2006.01) C12M 3/00 (2006.01) A61K 35/12 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2210449

The invention concerns a process for preparing a composition containing macrophages, if necessary activated, and/or cells derived from monocytes with high antigen presentation potential. The process includes a step in which monocytes present in the initial composition are cultured. This step is preceding and/or followed by a step during which all or some of the constituents other than the monocytes present in the initial composition are eliminated with the help of antibodies directed against these constitutents, and/or followed by elutriation. The invention includes the compositions and the kits for carrying out the process.

L'invention a pour objet un procédé de préparation d'une composition contenant des macrophages, le cas échéant activés, et/ou des cellules dérivées de monocytes à fort potentiel de présentation antigénique, ledit procédé comprenant une étape de mise en culture des monocytes présents dans la composition de départ, cette étape étant précédée et/ou suivie par une étape d'élimination de tout ou partie des constituants autres que les monocytes présents dans la composition de départ, à l'aide d'anticorps dirigés contre ces constituants, et/ou suivie par une étape d'élutriation. L'invention concerne également les compositions et les trousses pour la mise en oeuvre de ce procédé.

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