Method for preparing membrane vesicles

Details Method for preparing membrane vesicles Method for preparing membrane vesicles

A - Human Necessities

61

K

A61K 35/12 (2006.01) A61K 39/00 (2006.01) B01D 15/08 (2006.01) B01J 41/20 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2360752

The invention concerns a method for preparing membrane vesicles from a biological sample, characterised in that it comprises at least a step which consists in treating the sample by anion-exchange chromatography and/or gel permeation. The invention is used for preparing original vesicles or of different types, in particular from cells presenting antigens or tumour cells. The invention also concerns the resulting vesicles and their uses.

La présente invention concerne un procédé de préparation de vésicules membranaires à partir d'un échantillon biologique caractérisé en ce qu'il comprend au moins une étape de traitement de l'échantillon par chromatographie d'échange d'anions et/ou de perméation de gel. L'invention est utilisée pour la préparation de vésicules d'origines et de natures variées, notamment à partir de cellules présentatrices d'antigènes ou de cellules tumorales. L'invention concerne aussi les vésicules ainsi obtenues et leurs utilisations.

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