Method for preparing monoclonal antibody

Details Method for preparing monoclonal antibody Method for preparing monoclonal antibody

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) A01K 67/027 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2368734

A significantly increased amount of a monoclonal antibody is obtained from the culture medium of recombinant hybridoma prepared by introducing genes encoding a protein identical to the immunoglobulin heavy chain polypeptide of the specific monoclonal antibody into an immortalized B cell (hybridoma) producing the monoclonal antibody.

Selon l'invention, des hybridomes de recombinaison sont produits par transfert d'un gène, qui code une séquence d'acides aminés identique à celle du polypeptide à chaîne lourde d'un anticorps monoclonal spécifique, dans des lymphocytes B immortalisés (hybridomes) produisant cet anticorps monoclonal, ce qui permet d'obtenir ledit anticorps monoclonal dans des quantités très importantes, à partir du milieu de culture liquide desdits lymphocytes B.

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