C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2600240
A method for analysing a target polynucleotide having distinct units of nucleic acid sequence comprising: (i) forming a first polynucleotide which is a concatemer having multiple repeating target polynucleotide sequences; (ii) forming on the first polynucleotide a second polynucleotide hybridised to a portion of one or more of the target polynucleotides, such that the portion hybridised, or the portion not hybridised, corresponds to a sequence unit on the target, and determining the sequence unit on the target.
L'invention concerne une méthode d'analyse d'un polynucléotide cible présentant des unités distinctes de séquence d'acide nucléique. La méthode consiste à: i) former un premier polynucléotide qui est un concatémère constitué de multiples séquences répétées de polynucléotide cible; former sur le premier polynucléotide un second polynucléotide hybridé à une partie d'un ou de plusieurs polynucléotides cibles, de sorte que la partie hybridée, ou la partie non hybridée, corresponde à une unité de séquence sur la cible, et déterminer l'unité de séquence sur la cible.
Lingvitae As
Torys Llp
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