A - Human Necessities – 61 – L
Patent
A - Human Necessities
61
L
A61L 27/20 (2006.01) A61L 27/38 (2006.01) A61L 27/50 (2006.01) A61L 27/52 (2006.01) A61L 27/56 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2704738
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b). characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).
L'invention concerne un procédé permettant de préparer un échafaudage poreux pour l'ingénierie tissulaire. Elle concerne également un échafaudage poreux que l'on peut obtenir par ledit procédé, et son utilisation pour l'ingénierie tissulaire, la culture cellulaire et l'administration cellulaire. Ledit procédé consiste : a) à préparer une solution aqueuse alcaline comprenant une dose d'au moins un polysaccharide et un agent de réticulation, b) à congeler la solution aqueuse de a), c) à sublimer la solution congelée de b), ledit procédé se caractérisant en ce que b) est exécuté avant que la réticulation du polysaccharide se produise dans la solution en a).
Autissier Aude
Chaubet Frederic
Le Visage Catherine
Letourneur Didier
Inserm (institut National de La Sante Et de La Recherche Medical
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Universite Paris 7 - Denis Diderot
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