A - Human Necessities – 61 – L
Patent
A - Human Necessities
61
L
A61L 27/20 (2006.01) A61L 27/38 (2006.01) A61L 27/50 (2006.01) A61L 27/52 (2006.01) A61L 27/56 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2701858
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4°C to about 80°C for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).
L'invention concerne un procédé permettant de préparer un échafaudage poreux pour l'ingénierie tissulaire. L'objet concerne également un échafaudage poreux que l'on peut obtenir par ledit procédé, et son utilisation pour l'ingénierie tissulaire, la culture cellulaire et l'administration cellulaire. Ledit procédé consiste: a) préparer une solution aqueuse alcaline comprenant une dose d'au moins un polysaccharide, une dose d'un agent de réticulation et une dose d'un agent porogène, b) à transformer la solution en un hydrogel en plaçant ladite solution à une température comprise entre environ 4°C et environ 80°C pendant une durée suffisante pour permettre la réticulation de ladite dose de polysaccharide, et c) à immerger ledit hydrogel dans une solution aqueuse, d) à laver l'échafaudage poreux obtenu en c).
Le Visage Catherine
Letourneur Didier
Inserm (institut National de La Sante Et de La Recherche Medical
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Universite Paris 7 - Denis Diderot
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