G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/531 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/579 (2006.01) G01N 33/96 (2006.01)
Patent
CA 1208128
ABSTRACT OF THE DISCLOSURE (1) A method for preparing a sample for use in endotoxin test which comprises subjecting a sample of plasma or blood preparation to a contact with water- insoluble anti human .alpha.2-macroglobulin antibody and to a gel filtration by means of a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, and re- covering the maximum molecular weight fraction. (2) A kit for preparing a sample for use in endotoxin test consisting of a set of a sample-developing column prepared by packing a water-insoluble anti human .alpha.2-macroglobulin antibody and a gel of allyl dextran cross- linked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution of a high molecular colored substance to be added to the sample and a buffer solution for use in developing the sample.
387051
Kawasumi Keiichi
Maeda Shoichi
Shoji Takashi
Watanabe Ryozo
Fetherstonhaugh & Co.
Yoshitomi Pharmaceutical Industries Ltd.
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