Method for preparing single-stranded dna libraries

Details Method for preparing single-stranded dna libraries Method for preparing single-stranded dna libraries

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2513541

This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.

L'invention concerne des procédés de production de banques d'ADN simple brin destinées à être utilisées dans des réactions d'amplification et de séquençage. Selon divers aspects, lesdits procédés consistent: à fragmenter l'ADN; à polir les extrémités des fragments; à ligaturer les fragments à des adaptateurs universels; à procéder au déplacement de brin et à l'extension des fragments entaillés; à purifier les produits de ligature double brin; à capturer les produits de ligature double brin sur un support solide; et à isoler les fragments de la banque ADN simple brin, et à lier ces fragments à un autre support solide.

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