Method for preparing steroids modified by yeast fermentation

Details Method for preparing steroids modified by yeast fermentation Method for preparing steroids modified by yeast fermentation

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) A61K 31/56 (2006.01) C07J 75/00 (2006.01) C12N 1/19 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 15/54 (2006.01) C12P 33/06 (2006.01)

Patent

CA 2386419

The invention concerns a method for producing hydroxylated and/or acetylated steroid comprising steps which consist in: culturing yeasts on a medium comprising at least a precursor of such hydroxylated and/or acetylated steroids; then isolating the hydroxylated and/or acetylated steroids from the medium after bioconversion. Said method is characterised in that said yeasts are yeasts transformed so as to express the product of Cyp7b gene. The invention also concerns the modified yeast strains.

La présente invention concerne un procédé de production de stéroïde hydroxylé et/ou acétylé comprenant les étapes selon lesquelles: on cultive des levures sur un milieu comprenant au moins un précurseur de tels stéroïdes hydroxylés et/ou acétylés, puis on isole les stéroïdes hydroxylés et/ou acétylés du milieu après bioconversion, ledit procédé étant caractérisé en ce que lesdites levures sont des levures transformées de façon à exprimer le produit du gène Cyp7b. L'invention concerne aussi les souches de levures modifiées.

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