Method for preparing transformed plant

Details Method for preparing transformed plant Method for preparing transformed plant

A - Human Necessities

01

H

A01H 1/00 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2054607

A transformed plant of Gramineae is prepared by culturing an anther of Gramineae in a callus induction medium and, at a stage immediately before the microspore begins to cause division or during the initial division, transducing a genetic substance into the microspore cell through a pore formed by a laser pulse thereby to express genetic information of the genetic substance. According to the present invention, it is unnecessary to prepare protoplast and therefore, time and operations for transformation can be greatly reduced. Since haploid cells are transformed, the character transduced is conveyed without separating at a later generation. In addition, difficulties in experiments between species and strains are minimized so that it is easy to apply the present invention to practical species. According to the present invention, large pores can be formed as compared to the electroporation method so that DNA or substances having a large molecular weight can be introduced.

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