Method for preparing transgenic animal

Details Method for preparing transgenic animal Method for preparing transgenic animal

A - Human Necessities

61

K

A61K 48/00 (2006.01) A01K 67/02 (2006.01) A01K 67/027 (2006.01) A61D 19/02 (2006.01) A61K 9/127 (2006.01) A61K 31/70 (2006.01) C12N 15/87 (2006.01) C12N 15/88 (2006.01)

Patent

CA 2233694

A method for preparing spermatozoa having an alien DNA of a mature male nonhuman vertebrate transduced thereinto which comprises injecting the alien DNA carried by a liposome for transduction into the testes of the animal thrice or more repeatedly and thus producing spermatozoa having the alien DNA transduced thereinto in the testes of the animal. A transgenic nonhuman vertebrate can be conveniently and efficiently produced by fertilizing an unfertilized egg with such a spermatozoon by natural crossing, artificial fertilization or external fertilization.

Méthode de préparation de spermatozoïdes dans lesquels a été transduit un ADN étranger de vertébré non humain mâle mature. La méthode consiste à injecter l'ADN étranger véhiculé par un liposome de sorte qu'il soit transduit dans les testicules de l'animal trois fois ou plus de manière à obtenir dans les testicules de l'animal des spermatozoïodes présentant par transduction l'ADN étranger. Un vertébré transgénique non humain peut ainsi être obtenu commodément et efficacement en fécondant un oeuf non fécondé à l'aide d'un tel spermatozoïde par croisement naturel, fécondation artificielle ou fécondation externe.

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