Method for preserving polypeptides using a sugar and...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 1/00 (2006.01) A61K 47/26 (2006.01) C12N 9/96 (2006.01)

Patent

CA 2737407

A method for preserving a polypeptide comprises (i) providing an aqueous solution of one or more sugars, a polyethyleneimine and said polypeptide wherein the concentration of polyethyleneimine is 25 µM or less based on the number-average molar mass (Mn) of the polyethyleneimine and the sugar concentration or, if more than one sugar is present, total sugar concentration is greater than 0.1 M; and (ii) drying the solution to form an amorphous solid matrix comprising said polypeptide.

L'invention concerne un procédé de conservation d'un polypeptide, consistant : (i) à obtenir une solution aqueuse contenant au moins un sucre, une polyéthylèneimine et ledit polypeptide, la concentration de polyéthylèneimine étant de 25 µM ou inférieure, en fonction de la masse molaire moyenne en nombre (Mn) de la polyéthylèneimine, et la concentration de sucre ou, si plus d'un sucre est présent, la concentration totale de sucre étant supérieure à 0,1 M ; et (ii) à sécher la solution pour former une matrice solide amorphe contenant ledit polypeptide.

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