Method for preserving transplantable sheets of epithelium...

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01) A61L 15/16 (2006.01) A61L 27/00 (2006.01) A61L 27/60 (2006.01) C12N 5/02 (2006.01)

Patent

CA 1330541

- 18 - Abstract METHOD FOR PRESERVING TRANSPLANTABLE SHEETS OF EPITHELIUM CULTURED IN VITRO A process for preserving cultured epithelial sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide as cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.

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