Method for probing the function of a protein

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2161946

The present invention provides a method for an efficient elucidation of the function of the protein/eptide coded by cDNA in which only the partial sequence is elucidated and the function is unknown. The method for elucidating the function of the protein/peptide coded by cDNA in which only partial sequence is analyzed, characterized in that, changes in the biological function are evaluated when an antisense oligonucleotide complementary to the partial sequence of the cDNA wherein only partial sequence is analyzed is added to an evaluating system for the biological function whereby the protein or the peptide coded by said cDNA can be expressed. Function of the protein/peptide coded by cDNA can be efficiently elucidated even in a stage where only partial sequence of said cDNA is analyzed.

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