C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C07C 51/08 (2006.01) C12N 1/21 (2006.01) C12N 9/78 (2006.01) C12P 7/40 (2006.01)
Patent
CA 2626763
The present invention relates to a method for producing 5-norbornen-2- carboxylic acid from 5-norbornen-2-endo-carbonitrile and/or 5-norbornen-2-exo- carbonitrile. The invention relates more specifically to a method for producing 5-norbornen-2-carboxylic acid at a high substrate concentration. Furthermore, the invention relates to a polypeptide suitable for the enzymatic reaction of 5-norbornen-2-carbonitrile to 5-norbornen-2-carboxylic acid, particularly also at a high substrate concentration, as well as to a nucleic acid coding for the polypeptide, a composition containing 5-norbornen-2- carbonitrile to 5-norbornen-2-endo-carboxylic acid and 5-norbornen-2-exo- carboxylic acid, as well as the use of the polypeptide.
L'invention concerne un procédé de fabrication enzymatique d'acide 5-norbornène-2-carboxylique à partir de 5-norbornène-2-endo-carbonitrile et/ou de 5-norbornène-2-exo-carbonitrile. L'invention concerne notamment un procédé avec lequel de l'acide 5-norbornène-2-carboxylique peut être fabriqué à une concentration de substrat élevée. L'invention concerne également un polypeptide adapté à la transformation enzymatique de 5-norbornène-2-carbonitrile en acide 5-norbornène-2-carboxylique, notamment à une concentration de substrat élevée, ainsi qu'un acide nucléique codant le polypeptide, une composition contenant du 5-norbornène-2-carbonitrile transformé en acide 5-norbornène-2-endo-carboxylique et de l'acide 5-norbornène-2-exo-carboxylique, et l'utilisation du polypeptide.
Hauer Bernhard
Kesseler Maria
Basf Se
Robic
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