Method for producing a compound of interest in a filamentous...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/80 (2006.01) C12N 1/15 (2006.01) C12P 1/02 (2006.01) C12N 9/20 (2006.01) C12N 9/30 (2006.01)

Patent

CA 2595056

The invention relates to a nucleotide sequence comprising: a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, said synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally said nucleotide sequence comprises control sequences such as: one translational termination sequence orientated in 5' towards 3' direction selected from the following list of sequences: TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5' towards 3' direction selected from the following list of sequences: gctnccyyc, using ambiguity codes for nucleotides: v (A/C/G); n (A/C/G/T), preferably 5'- GCT TCC TTC-3'. The invention further relates to a consensus translational initiator sequence: 5'-mwChkyCAmv-3', preferably the translational initiator sequence is selected from the list consisting of: 5'-mwChkyCAAA-3', 5'- mwChkyCACA-3', and 5'-mwChkyCAAG-3'.

L'invention porte sur une séquence nucléotidique comprenant une séquence de codage nucléotidique synonyme présentant une fréquence codon synonyme, ce codon synonyme codant le même acide aminé que le codon natif et présentant une fréquence plus élevée dans l'usage codon tel que défini dans le tableau 1 que le codon natif. Facultativement, cette séquence nucléotidique comprend des séquences de contrôle telles qu'une séquence de terminaison translationnelle orientée dans le sens 5' vers 3' choisie dans la liste de séquences suivantes : TAAG, TAGA et TAAA, de préférence TAAA, et/ou une séquence de codage initial translationnelle orientée dans le sens 5' vers 3' choisie dans la liste de séquences suivante : gctnccyyc, au moyen des codes d'ambiguïté pour des nucléotides : v(A/C/G); n (A/C/G/T), de préférence 5'-GCT TCC TTC-3'. Cette invention porte aussi sur une séquence initiale translationnelle consensus : 5'-mwChkyCAmv-3', la séquence d'initiateur translationnelle étant choisie de préférence dans la liste: 5'-mwChkyCAAA-3', 5'-mwChkyCACA-3', et 5'-mwChkyCAAG-3'.

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