Method for producing a marker-free mutated target organism...

Details Method for producing a marker-free mutated target organism... Method for producing a marker-free mutated target organism...

C - Chemistry, Metallurgy

12

N

C12N 15/77 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2456222

The invention relates to a plasmid vector which does not replicate in a target organism and contains the following constituents: a) an origin of replication for a host organism which is not identical to the target organism, b) at least one genetic marker, c) optionally a section of a sequence which enables the transfer of DNA by means of conjugation (mob sequence), d) a section of a sequence which is homologous to sequences of the target organism and enables homologous recombination in the target organism, e) a gene for a galactokinase under the control of a promoter.

L'invention concerne un vecteur plasmidique qui n'est pas répliqué dans un organisme cible, et contenant les composants suivants : a) une origine de réplication d'un organisme hôte qui est identique à l'organisme cible ; b) au moins un gène marqueur ; c) éventuellement une section de séquence qui permet le transfert d'ADN par conjugaison (séquence mob) ; d) une section de séquence qui est homologue à des séquences de l'organisme cible et qui permet une recombinaison homologue dans l'organisme cible ; e) un gène de galactose kinase sous contrôle d'un promoteur.

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