Method for producing a normalized gene library from nucleic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/63 (2006.01) C12N 15/10 (2006.01) C12N 15/66 (2006.01) C12Q 1/68 (2006.01) G01N 33/24 (2006.01)

Patent

CA 2461139

The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

L'invention se rapporte à un procédé de production d'une banque de gènes normalisée à partir d'extraits d'acides nucléiques provenant d'échantillons de sol, ainsi qu'aux structures de gènes et vecteurs auxquels ce procédé fait appel. L'invention concerne en outre l'utilisation de ladite banque de gènes normalisée pour cribler des gènes codant pour de nouveaux biocatalyseurs provenant d'échantillons de sol.

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