Method for producing a recombinant baculovirus expression...

Details Method for producing a recombinant baculovirus expression... Method for producing a recombinant baculovirus expression...

C - Chemistry, Metallurgy

12

N

195/129, 195/1.2

C12N 15/00 (2006.01) C07K 14/01 (2006.01) C07K 14/52 (2006.01) C12N 15/866 (2006.01)

Patent

CA 1222213

METHOD FOR PRODUCING A RECOMBINANT BACULOVIRUS EXPRESSION VECTOR ABSTRACT A method for producing a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer fector is contacted with a baculo- virus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.

455167

Affiliated with

Also associated with

Rating

Method for producing a recombinant baculovirus expression... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing a recombinant baculovirus expression..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing a recombinant baculovirus expression... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1186415