Method for producing a recombinant protein on a...

Details Method for producing a recombinant protein on a... Method for producing a recombinant protein on a...

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C12N 15/10 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2687170

A method for producing a protein of interest on a manufacturing scale is based on integration,by homologous recombination, of the DNA encoding the protein of interest into a bacterial cell sgenome at a pre-selected site. The manufacturing scale production of recombinant proteins is in the fed-batch mode, semi-continuous or in a chemostat.

La présente invention concerne un procédé de production d'une protéine d'intérêt à l'échelle industrielle basé sur l'intégration, par recombinaison homologue, de l'ADN codant pour la protéine d'intérêt dans le génome d'une cellule bactérienne dans un site sélectionné à l'avance. La production à l'échelle industrielle des protéines recombinées s'effectue en mode à alimentation continue, en mode à alimentation discontinue ou dans un chémostat.

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