Method for producing a soluble protein with bacteria

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/21 (2006.01) C07K 14/245 (2006.01) C12N 15/70 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2187250

A co-transformed bacterium with both an expression vector for a thioredoxin gene and an expression vector for a desired gene; and a method for producing a soluble protein by cultivating the co-transformed bacterium to produce a protein encoded by a desired gene and recovering the protein as a soluble protein.

Bactérie co-transformée avec à la fois un vecteur d'expression pour un gène de thiorédoxine et un vecteur d'expression pour un gène souhaité; méthode pour produire une protéine soluble par culture de la bactérie co-transformée de façon à obtenir une protéine codée par un gène souhaité, et récupération de la protéine sous forme de protéine soluble.

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