Method for producing alcohol in a bio-refinery environment

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 7/10 (2006.01) C12F 3/10 (2006.01) C12N 9/42 (2006.01) C12P 1/02 (2006.01)

Patent

CA 2704215

The invention relates to a method for producing alcohol from a pre-processed lignocellulosic biomass, in which the enzymatic hydrolysis step is carried out with cellulolytic and/or semicellulolytic enzymes generated by using at least one effluent resulting from another ethanol production method using a sugar plant as a raw material. This method can be integrated into a method for producing alcohol from so-called second-generation cellulosic or lignocellulosic materials, that comprises the following steps: 1) the chemical and/or physical pre-processing of a cellulosic or lignocellulosic substrate; 2) the enzymatic hydrolysis of the pre-processed substrate using cellulolytic and/or semicellulolytic enzymes; the alcoholic fermentation by an appropriate alcohol-producing microorganism of the hydrolysate from step 2) and the production of a fermentation wort; and the separation of the alcohol-producing microorganism used in step 3), with the separation/purification of the alcohol.

La présente invention décrit un procédé de production d'alcool à partir de biomasse lignocellulosique prétraitée dans lequel l'étape d'hydrolyse enzymatique est réalisée avec des enzymes cellulolytiques et/ou hémicellulolytiques produites en utilisant au moins un effluent issu d'un autre procédé de production d'éthanol utilisant comme matière première une plante sucrière. Ce procédé peut être intégré dans un procédé de production d'alcool à partir de matériaux cellulosiques ou ligno-- cellulosiques, dit de seconde génération qui comprend les étapes suivantes : 1) le prétraitement chimique et/ou physique d'un substrat cellulosique ou ligno- cellulosique; 2) l'hydrolyse enzymatique du substrat prétraité utilisant des enzymes cellulolytiques et/ou hémicellulolytiques; 3) la fermentation alcoolique par un microorganisme alcooligène approprié de l'hydrolysat issu de l'étape (2) et obtention d'un moût de fermentation; et la séparation du micro- organisme alcooligène utilisé dans l'étape (3), la séparation/ purification de l'alcool.

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